A single cytochrome P450 oxidase from<i>Solanum habrochaites</i>sequentially oxidizes 7?<i>epi</i>?zingiberene to derivatives toxic to whiteflies and various microorganisms

Secretions from glandular trichomes potentially protect plants against a variety of aggressors. In the tomato clade Solanum genus, wild species produce rich source chemical diversity at leaf surface. Previously, 7-epi-zingiberene produced in several accessions habrochaites was found to confer resistance whiteflies (Bemisia tabaci) and other insect pests. Here, we report identification characterisation 9-hydroxy-zingiberene (9HZ) 9-hydroxy-10,11-epoxyzingiberene (9H10epoZ), two derivatives S. LA2167. Using combination transcriptomics genetics, identified gene coding for cytochrome P450 oxygenase, ShCYP71D184, that is highly expressed co-segregates with presence zingiberene derivatives. Transient expression assays Nicotiana benthamiana showed ShCYP71D184 carries out successive oxidations generate 9HZ 9H10epoZ. Bioactivity particular exhibits substantial toxicity B. tabaci various microorganisms including Phytophthora infestans Botrytis cinerea. Our work shows trichome secretions can provide protection wide organisms. addition, availability genes encoding enzymes pathway makes it possible introduce this trait cultivated by precision breeding.